Protocols

Click on a link below to download and view a PDF version of the listed Protocols.

Aflatoxin Analysis

• Thin Layer Chromatography (TLC) Analysis
• TLC for Aflatoxin

Array Protocols

• SOP - cDNA Microarray
• RNA_conc. (Excel Spreadsheet)
• The Institute For Genomic Research Standard Operating Procedure

DNA Analysis

• Prep of Genomic DNA – FUNGI
• CTAB Method for Colony Preparation

Fungal Transformation

A. flavus

• A. flavus Transformation

A. nidulans

• Protoplast Isolation and Transformation
• Protoplast Preparation for A. nidulans
• Protoplast Preparation
• Transformation of A. fumigatus by Electroporation
• Transformation Using Protoplasts

Media

• Modified A&M Media
• SLS Medium

RNA

• Formaldehyde Gels
• RNA Preparation from A. parasiticus
• RNA prep Protocols
• Kernel RNA prep Protocols